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cleaved casp1  (Proteintech)


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    Structured Review

    Proteintech cleaved casp1
    Cleaved Casp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+casp1/Caspase+1%2Fp20%2Fp10+Antibody/pmc12766190-196-31-55
    Average 96 stars, based on 1685 article reviews
    cleaved casp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Expressing:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Control:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    TUNEL Assay:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Staining:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Western Blot:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Quantitative RT-PCR:

    Article Title: Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion
    Article Snippet: Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.Finally, 5 % goat serum was added to block the cell slides for 30 min at room temperature, and the primary antibody was applied and incubated at 4 °C overnight.. The primary antibody used: ZBP1 (cat. sc-271483, Santa Cruz, USA), GM130 (cat. A25013 , Abclonal, China), cleaved-casp3 (cat. 9661, CST, USA), p-MLKL (cat. AF7420, Affinity, China), p-MLKL (cat. 37333, CST, USA), cleaved casp1 (cat. AF4022, Affinity, China), NF-κB (cat. 8242, CST, USA), CD68 (cat. 97778, CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA).. The following day, tissue sections and cell slides were processed in a similar manner.The following day, tissue sections and cell slides were processed in a similar manner.

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr

    Article Title: Bioinformatics analysis and validation of novel biomarkers and competitive endogenous RNA networks involved in pyroptosis in diabetic nephropathy.
    Article Snippet: equal amount of protein samples from rats in each group (n = 6) were separated using 10% SDS-PAGE. .. The blots were probed with primary antibodies for GAPDH (1:5000, Proteintech, 10494-1-AP), NLRP3 (1:1500, Proteintech, 27458-1-AP), pro-casp1 (1:1500, Affinity, AF5418), cleaved-casp1 (1:1000, Affinity, AF4005), GSDMD (1:1500, Affinity, AF4012), and GSDMD-N (1:1500, Affinity, AF4012), IRF9(1:1000, Proteintech, 14167-1-AP) , TXNIP (1:1000, Proteintech, 18243-1-AP), TRAF3(1:3000, Proteintech, 18099-1-AP). .. The bands were visualized using an enhanced chemiluminescence reagent (Affinity, KF005), and image pr



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    Figure 1. Whey protein hydrolysate (WPH) alleviated acetaminophen (APAP)-induced liver injury and pyroptosis in mice. (A) Hematoxylin and eosin staining of liver tissue in drug-induced liver injury (DILI) mice (scale bar = 100 μm). (B) Serum Ala aminotransferase (ALT) and aspartate aminotransferase (AST) content were measured by kits in mice. (C) Serum IL-1β and -18 levels in mice. (D) Liver/BW of mice. (E and F) The protein expression levels of NLRP3, GSDMD-N, cleaved <t>CASP1,</t> IL-1β, and -18. (G) Immunofluorescence staining of NLRP3 in mice (scale bar = 20 μm). Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP. **P < 0.01, #P < 0.05, and ##P < 0.01. GSDMD-N = GSDMD N-terminal fragment.
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    Image Search Results


    Figure 1. Whey protein hydrolysate (WPH) alleviated acetaminophen (APAP)-induced liver injury and pyroptosis in mice. (A) Hematoxylin and eosin staining of liver tissue in drug-induced liver injury (DILI) mice (scale bar = 100 μm). (B) Serum Ala aminotransferase (ALT) and aspartate aminotransferase (AST) content were measured by kits in mice. (C) Serum IL-1β and -18 levels in mice. (D) Liver/BW of mice. (E and F) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. (G) Immunofluorescence staining of NLRP3 in mice (scale bar = 20 μm). Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP. **P < 0.01, #P < 0.05, and ##P < 0.01. GSDMD-N = GSDMD N-terminal fragment.

    Journal: Journal of dairy science

    Article Title: Whey protein hydrolysate alleviated acetaminophen-induced hepatocyte pyroptosis by activating mitophagy.

    doi: 10.3168/jds.2025-26625

    Figure Lengend Snippet: Figure 1. Whey protein hydrolysate (WPH) alleviated acetaminophen (APAP)-induced liver injury and pyroptosis in mice. (A) Hematoxylin and eosin staining of liver tissue in drug-induced liver injury (DILI) mice (scale bar = 100 μm). (B) Serum Ala aminotransferase (ALT) and aspartate aminotransferase (AST) content were measured by kits in mice. (C) Serum IL-1β and -18 levels in mice. (D) Liver/BW of mice. (E and F) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. (G) Immunofluorescence staining of NLRP3 in mice (scale bar = 20 μm). Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP. **P < 0.01, #P < 0.05, and ##P < 0.01. GSDMD-N = GSDMD N-terminal fragment.

    Article Snippet: The antibodies for cleaved CASP1 (AF4005), PINK1 (DF7742), and PRKN (AF0235) were provided by Affinity Biosciences.

    Techniques: Staining, Expressing, Immunofluorescence, Control

    Figure 2. Whey protein hydrolysate (WPH) alleviated acetaminophen (APAP)-induced pyroptosis in AML-12 cells. (A) Cell viability was deter- mined by MTT assay. (B) Propidium iodide (PI) staining in AML-12 cells (scale bar = 100 μm). (C) Release of lactate dehydrogenase (LDH) from cell supernatants. (D) Levels of IL-1β and -18 in cell supernatants. (E and F) The protein expression levels of NLRP3, GSDMD N-terminal fragment (GSDMD-N), cleaved CASP1, IL-1β, and -18. (G) Immunofluorescence staining of NLRP3 in AML-12 cells (scale bar = 20 μm). Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP. **P < 0.01, #P < 0.05, and ##P < 0.01.

    Journal: Journal of dairy science

    Article Title: Whey protein hydrolysate alleviated acetaminophen-induced hepatocyte pyroptosis by activating mitophagy.

    doi: 10.3168/jds.2025-26625

    Figure Lengend Snippet: Figure 2. Whey protein hydrolysate (WPH) alleviated acetaminophen (APAP)-induced pyroptosis in AML-12 cells. (A) Cell viability was deter- mined by MTT assay. (B) Propidium iodide (PI) staining in AML-12 cells (scale bar = 100 μm). (C) Release of lactate dehydrogenase (LDH) from cell supernatants. (D) Levels of IL-1β and -18 in cell supernatants. (E and F) The protein expression levels of NLRP3, GSDMD N-terminal fragment (GSDMD-N), cleaved CASP1, IL-1β, and -18. (G) Immunofluorescence staining of NLRP3 in AML-12 cells (scale bar = 20 μm). Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP. **P < 0.01, #P < 0.05, and ##P < 0.01.

    Article Snippet: The antibodies for cleaved CASP1 (AF4005), PINK1 (DF7742), and PRKN (AF0235) were provided by Affinity Biosciences.

    Techniques: MTT Assay, Staining, Expressing, Immunofluorescence, Control

    Figure 5. Whey protein hydrolysate (WPH) alleviated pyroptosis by activating mitophagy in mice liver. (A) Fluorescence images of reactive oxygen species (ROS) in mouse primary cells (scale bar = 50 μm). (B) Immunofluorescence colocalization of NLRP3 and CTSB (scale bar = 20 μm). (C) The protein expression levels of LC3, P62, PRKN, and PINK1. (D) Immunofluorescence colocalization of LC3 and PINK1 (scale bar = 20 μm). (E) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP; arrow point (˄) versus APAP + WPH. *P < 0.05, **P < 0.01, #P < 0.05, ##P < 0.01, ˄P < 0.05, ˄˄P < 0.01.

    Journal: Journal of dairy science

    Article Title: Whey protein hydrolysate alleviated acetaminophen-induced hepatocyte pyroptosis by activating mitophagy.

    doi: 10.3168/jds.2025-26625

    Figure Lengend Snippet: Figure 5. Whey protein hydrolysate (WPH) alleviated pyroptosis by activating mitophagy in mice liver. (A) Fluorescence images of reactive oxygen species (ROS) in mouse primary cells (scale bar = 50 μm). (B) Immunofluorescence colocalization of NLRP3 and CTSB (scale bar = 20 μm). (C) The protein expression levels of LC3, P62, PRKN, and PINK1. (D) Immunofluorescence colocalization of LC3 and PINK1 (scale bar = 20 μm). (E) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP; arrow point (˄) versus APAP + WPH. *P < 0.05, **P < 0.01, #P < 0.05, ##P < 0.01, ˄P < 0.05, ˄˄P < 0.01.

    Article Snippet: The antibodies for cleaved CASP1 (AF4005), PINK1 (DF7742), and PRKN (AF0235) were provided by Affinity Biosciences.

    Techniques: Fluorescence, Immunofluorescence, Expressing, Control

    Figure 6. Whey protein hydrolysate (WPH) alleviated pyroptosis by activating mitophagy in AML-12 cells. (A) Fluorescence images of mito- chondrial membrane potential (MMP) in AML-12 cells (scale bar = 50 μm). (B) Immunofluorescence colocalization of NLRP3 and CTSB (scale bar = 20 μm). (C) Fluorescent and flow cytometric maps of reactive oxygen species (ROS) and mitochondrial (mt)ROS in AML-12 cells (scale bar = 50 μm). (D) The protein expression levels of LC3, P62, PRKN, and PINK1. (E) Immunofluorescence colocalization of LC3 and MitoTracker (scale bar = 20 μm). (F) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP; arrow point (˄) versus APAP + WPH. *P < 0.05, **P < 0.01; #P < 0.05, ##P < 0.01; ˄P < 0.05, ˄˄P < 0.01.

    Journal: Journal of dairy science

    Article Title: Whey protein hydrolysate alleviated acetaminophen-induced hepatocyte pyroptosis by activating mitophagy.

    doi: 10.3168/jds.2025-26625

    Figure Lengend Snippet: Figure 6. Whey protein hydrolysate (WPH) alleviated pyroptosis by activating mitophagy in AML-12 cells. (A) Fluorescence images of mito- chondrial membrane potential (MMP) in AML-12 cells (scale bar = 50 μm). (B) Immunofluorescence colocalization of NLRP3 and CTSB (scale bar = 20 μm). (C) Fluorescent and flow cytometric maps of reactive oxygen species (ROS) and mitochondrial (mt)ROS in AML-12 cells (scale bar = 50 μm). (D) The protein expression levels of LC3, P62, PRKN, and PINK1. (E) Immunofluorescence colocalization of LC3 and MitoTracker (scale bar = 20 μm). (F) The protein expression levels of NLRP3, GSDMD-N, cleaved CASP1, IL-1β, and -18. Data are presented as means ± SD. Asterisk (*) versus Control; pound sign (#) versus APAP; arrow point (˄) versus APAP + WPH. *P < 0.05, **P < 0.01; #P < 0.05, ##P < 0.01; ˄P < 0.05, ˄˄P < 0.01.

    Article Snippet: The antibodies for cleaved CASP1 (AF4005), PINK1 (DF7742), and PRKN (AF0235) were provided by Affinity Biosciences.

    Techniques: Fluorescence, Membrane, Immunofluorescence, Expressing, Control